Restrictions in blood flow and elevated platelet counts increase the risk of developing thrombosis or bleeding. Using the innovative 'flow chamber' method developed at the Department of Biochemistry at Maastricht University in the Netherlands, Coagulation Profile BV offers expertise in the application of the results of flow chamber tests for basic research projects and pre-clinical and clinical studies.
Global Haemostasis Assays
Whole blood global assays include both coagulation plasma components and platelets, providing a suitable in vitro system for assessing overall haemostasis. Coagulation profile offers coagulation measurements under Flow conditions according to customers’ needs and conditions. Two research systems are available at Coagulation Profile for coagulation measurements under different flow conditions:
T-TAS (Total Thrombus formation Analysis System) is a system that creates and analyzes thrombus formation under blood flow conditions using a disposable microchip with micro-level paths. When performing analysis using T-TAS, whole blood is passed through the chip, allowing the rigidity of the formed thrombus to be measured in the form of pressure. A chronological pressure waveform graph can be obtained from the T-TAS measurement results, allowing comprehensive total thrombus formation to be evaluated by analyzing the graph and comparing the calculated parameters. Platelet number/function and related fibrin clotting can be measured in blood, influenced by platelet inhibitors, coagulation inhibitors and fibrinolysis activators. Three different types of chips are available to measure under different shear conditions and/or different paths (capillary) coating.
Maastricht Flow Chamber is an in vitro flow chamber technique by which the role of platelets in regulation of thrombus formation can be investigated in interaction with the blood plasma, leukocytes and vessel wall components. Platelets play a crucial role in hemostasis and thrombosis and their improper function can lead to either bleeding tendencies or occlusive thrombus formation. Platelet binding, thrombus formation and platelet receptor expression after platelet activation can be studied by using fluorescent labeled antibodies. Fibrin formation can be studied, simultaneously, with fluorescent dyes. Since this system works with chambers which can be coated with multiple reagents, a wide field of research can be explored taking into account custom needs and preferred assay conditions.